1X9W

T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-ATP as the incoming nucleotide.

Summary for 1X9W

Related1T7P 1X9M 1X9S
Descriptor5'-D(*GP*GP*AP*GP*AP*GP*TP*GP*AP*TP*T*GP*GP*T*AP*GP*TP*GP*TP*GP*AP*(2DT))-3', 5'-D(*CP*CP*CP*(AFG)*AP*TP*CP*AP*CP*AP*CP*TP*AP*CP*CP*AP*AP*TP*CP*AP*CP*TP*CP*TP*CP*C)-3', DNA polymerase, ... (6 entities in total)
Functional Keywordsdna polymerase, n-2-aminofluorene, mutagenesis, replication block, transferase-electron transport-dna complex, transferase/electron transport/dna
Biological sourceEnterobacteria phage T7
More
Total number of polymer chains4
Total molecular weight105664.21
Authors
Dutta, S.,Li, Y.,Johnson, D.,Dzantiev, L.,Richardson, C.C.,Romano, L.J.,Ellenberger, T. (deposition date: 2004-08-24, release date: 2004-10-26, Last modification date: 2011-07-13)
Primary citation
Dutta, S.,Li, Y.,Johnson, D.,Dzantiev, L.,Richardson, C.C.,Romano, L.J.,Ellenberger, T.
Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis.
Proc.Natl.Acad.Sci.Usa, 101:16186-16191, 2004
PubMed: 15528277 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.0406516101
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
NMR Information
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.269111.7%3.2%7.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report