1X9Q

4m5.3 anti-fluorescein single chain antibody fragment (scFv)

Summary for 1X9Q

Entry DOI	10.2210/pdb1x9q/pdb
Descriptor	4m5.3 anti-fluorescein single chain antibody fragment, ACETATE ION, 2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-BENZOIC ACID, ... (4 entities in total)
Functional Keywords	very high affinity, antibody binding, electrostatics, directed evolution, single chain antibody, immune system
Biological source	Homo sapiens (human)
Total number of polymer chains	1
Total formula weight	30110.27
Authors	Midelfort, K.S.,Hernandez, H.H.,Lippow, S.M.,Tidor, B.,Drennan, C.L.,Wittrup, K.D. (deposition date: 2004-08-24, release date: 2005-03-22, Last modification date: 2023-08-23)
Primary citation	Midelfort, K.S.,Hernandez, H.H.,Lippow, S.M.,Tidor, B.,Drennan, C.L.,Wittrup, K.D. Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody J.Mol.Biol., 343:685-701, 2004 Cited by PubMed: 15465055 DOI: 10.1016/j.jmb.2004.08.019 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.5 Å)

Structure validation