1WV1

Crystallographic studies on acyl ureas, a new class of inhibitors of glycogenphosphorylase. Broad specificity of the allosteric site

Summary for 1WV1

• Entry DOI: 10.2210/pdb1wv1/pdb
• Related: 1WUT 1WUY 1WV0 3AMV
• Descriptor: Glycogen phosphorylase, muscle form, PYRIDOXAL-5'-PHOSPHATE, 5-[3-({[(2,4-DICHLOROBENZOYL)AMINO]CARBONYL}AMINO)-2-METHYLPHENOXY]PENTANOIC ACID, ... (4 entities in total)
• Functional Keywords: glycogenolysis, type 2 diabetes, transferase
• Biological source: Oryctolagus cuniculus (rabbit)
• Total number of polymer chains: 1
• Total formula weight: 97977.63

Authors

Oikonomakos, N.G.,Kosmopoulou, M.N.,Chrysina, E.D.,Leonidas, D.D.,Klabunde, T.,Wendt, K.U.,Defossa, E. (deposition date: 2004-12-10, release date: 2005-12-10, Last modification date: 2011-07-13)

Primary citation

Oikonomakos, N.G.,Kosmopoulou, M.N.,Chrysina, E.D.,Leonidas, D.D.,Kostas, I.D.,Wendt, K.U.,Klabunde, T.,Defossa, E.
Crystallographic studies on acyl ureas, a new class of glycogen phosphorylase inhibitors, as potential antidiabetic drugs
Protein Sci., 14:1760-1771, 2005

PubMed Abstract: Acyl ureas were discovered as a novel class of inhibitors for glycogen phosphorylase, a molecular target to control hyperglycemia in type 2 diabetics. This series is exemplified by 6-{2,6-Dichloro- 4-[3-(2-chloro-benzoyl)-ureido]-phenoxy}-hexanoic acid, which inhibits human liver glycogen phosphorylase a with an IC(50) of 2.0 microM. Here we analyze four crystal structures of acyl urea derivatives in complex with rabbit muscle glycogen phosphorylase b to elucidate the mechanism of inhibition of these inhibitors. The structures were determined and refined to 2.26 Angstroms resolution and demonstrate that the inhibitors bind at the allosteric activator site, where the physiological activator AMP binds. Acyl ureas induce conformational changes in the vicinity of the allosteric site. Our findings suggest that acyl ureas inhibit glycogen phosphorylase by direct inhibition of AMP binding and by indirect inhibition of substrate binding through stabilization of the T' state.

PubMed: 15987904
DOI: 10.1110/ps.051432405

Experimental method

X-RAY DIFFRACTION (2.26 Å)