1WPO
HYDROLYTIC ENZYME HUMAN CYTOMEGALOVIRUS PROTEASE
Summary for 1WPO
| Entry DOI | 10.2210/pdb1wpo/pdb |
| Descriptor | HUMAN CYTOMEGALOVIRUS PROTEASE, SULFATE ION (3 entities in total) |
| Functional Keywords | coat protein, hydrolase, serine protease, phosphorylation, viral protease, viral protein |
| Biological source | Human herpesvirus 5 (Human cytomegalovirus) |
| Cellular location | Protease precursor: Host cytoplasm. Assemblin: Host nucleus. Assembly protein: Host nucleus: P16753 |
| Total number of polymer chains | 2 |
| Total formula weight | 56620.68 |
| Authors | Tong, L. (deposition date: 1996-07-23, release date: 1997-10-15, Last modification date: 2024-10-30) |
| Primary citation | Tong, L.,Qian, C.,Massariol, M.J.,Bonneau, P.R.,Cordingley, M.G.,Lagace, L. A new serine-protease fold revealed by the crystal structure of human cytomegalovirus protease. Nature, 383:272-275, 1996 Cited by PubMed Abstract: Human cytomegalovirus (hCMV), a herpesvirus, infects up to 70% of the general population in the United States and can cause morbidity and mortality in immunosuppressed individuals (organ-transplant recipients and AIDS patients) and congenitally infected newborns. hCMV protease is essential for the production of mature infectious virions, as it performs proteolytic processing near the carboxy terminus (M-site) of the viral assembly protein precursor. hCMV protease is a serine protease, although it has little homology to other clans of serine proteases. Here we report the crystal structure of hCMV protease at 2.0 angstroms resolution, and show that it possesses a new polypeptide backbone fold. Ser 132 and His 63 are found in close proximity in the active site, confirming earlier biochemical and mutagenesis studies. The structure suggests that the third member of the triad is probably His 157. A dimer of the protease with an extensive interface is found in the crystal structure. This structure information will help in the design and optimization of inhibitors against herpesvirus proteases. PubMed: 8805706DOI: 10.1038/383272a0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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