1WPI

Solution NMR Structure of Protein YKR049C from Saccharomyces cerevisiae. Ontario Centre for Structural Proteomics target YST0250_1_133; Northeast Structural Genomics Consortium YTYst250

Summary for 1WPI

• Entry DOI: 10.2210/pdb1wpi/pdb
• Descriptor: Hypothetical 15.6 kDa protein in NAP1-TRK2 intergenic region (1 entity in total)
• Functional Keywords: structural genomics, ocsp, nesg, protein structure initiative, psi-2, northeast structural genomics consortium, unknown function
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Mitochondrion: P36141
• Total number of polymer chains: 1
• Total formula weight: 15672.04

Authors

Jung, J.W.,Yee, A.,Arrowsmith, C.H.,Lee, W.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2004-09-03, release date: 2005-09-13, Last modification date: 2024-05-29)

Primary citation

Jung, J.W.,Yee, A.,Wu, B.,Arrowsmith, C.H.,Lee, W.
Solution structure of YKR049C, a putative redox protein from Saccharomyces cerevisiae
J.Biochem.Mol.Biol., 38:550-554, 2005

PubMed Abstract: YKR049C is a mitochondrial protein in Saccharomyces cerevisiae that is conserved among yeast species, including Candida albicans. However, no biological function for YKR049C has been ascribed based on its primary sequence information. In the present study, NMR spectroscopy was used to determine the putative biological function of YKR049C based on its solution structure. YKR049C shows a well-defined thioredoxin fold with a unique insertion of helices between two beta-strands. The central beta-sheet divides the protein into two parts; a unique face and a conserved face. The 'unique face' is located between beta2 and beta3. Interestingly, the sequences most conserved among YKR049C families are found on this 'unique face', which incorporates L109 to E114. The side chains of these conserved residues interact with residues on the helical region with a stretch of hydrophobic surface. A putative active site composed by two short helices and a single Cys97 was also well observed. Our findings suggest that YKR049C is a redox protein with a thioredoxin fold containing a single active cysteine.

PubMed: 16202234

Experimental method

SOLUTION NMR