1WCD

Crystal structure of IBDV T1 virus-like particle reveals a missing link in icosahedral viruses evolution

Summary for 1WCD

• Entry DOI: 10.2210/pdb1wcd/pdb
• Related: 1WCE
• Descriptor: MAJOR STRUCTURAL PROTEIN VP2 (2 entities in total)
• Functional Keywords: virus, non-enveloped icosahedral viruses, double-stranded rna virus protein, birnavirus, transcriptase machinery, hydrolase, membrane translocation activity, evolution, icosahedral virus
• Biological source: INFECTIOUS BURSAL DISEASE VIRUS (IBDV)
• Cellular location: Capsid protein VP2: Virion (Potential). Capsid protein VP3: Virion (Potential). Structural peptide 1: Virion (Potential). Structural peptide 2: Virion (Potential). Structural peptide 3: Virion (Potential). Structural peptide 4: Virion (Potential): P15480
• Total number of polymer chains: 1
• Total formula weight: 47192.11

Authors

Coulibaly, F.,Chevalier, C.,Gutsche, I.,Pous, J.,Bressanelli, S.,Navaza, J.,Delmas, B.,Rey, F.A. (deposition date: 2004-11-12, release date: 2005-04-05, Last modification date: 2024-05-08)

Primary citation

Coulibaly, F.,Chevalier, C.,Gutsche, I.,Pous, J.,Navaza, J.,Bressanelli, S.,Delmas, B.,Rey, F.A.
The Birnavirus Crystal Structure Reveals Structural Relationships Among Icosahedral Viruses.
Cell(Cambridge,Mass.), 120:761-, 2005

PubMed Abstract: Double-stranded RNA virions are transcriptionally competent icosahedral particles that must translocate across a lipid bilayer to function within the cytoplasm of the target cell. Birnaviruses are unique among dsRNA viruses as they have a single T = 13 icosahedral shell, lacking the characteristic inner capsid observed in the others. We determined the crystal structures of the T = 1 subviral particle (260 angstroms in diameter) and of the T = 13 intact virus particle (700 angstroms in diameter) of an avian birnavirus to 3 angstroms and 7 angstroms resolution, respectively. Our results show that VP2, the only component of the virus icosahedral capsid, is homologous both to the capsid protein of positive-strand RNA viruses, like the T = 3 nodaviruses, and to the T = 13 capsid protein of members of the Reoviridae family of dsRNA viruses. Together, these results provide important insights into the multiple functions of the birnavirus capsid and reveal unexpected structural relationships among icosahedral viruses.

PubMed: 15797378
DOI: 10.1016/J.CELL.2005.01.009

Experimental method

X-RAY DIFFRACTION (3 Å)