1WA8

Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria

1WA8 の概要

• エントリーDOI: 10.2210/pdb1wa8/pdb
• 分子名称: ESAT-6 LIKE PROTEIN ESXB, 6 KDA EARLY SECRETORY ANTIGENIC TARGET (ESAT-6) (2 entities in total)
• 機能のキーワード: tuberculosis, cfp-10, esat-6, helix-turn-helix, four helix bundle, mycobacteria, pathogenesis, solution structure, psi, protein structure initiative, tb structural genomics consortium, tbsgc
• 由来する生物種: MYCOBACTERIUM BOVIS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20580.35

構造登録者

Renshaw, P.S.,Lightbody, K.L.,Veverka, V.,Muskett, F.W.,Kelly, G.,Frenkiel, T.A.,Gordon, S.V.,Hewinson, R.G.,Burke, B.,Norman, J.,Williamson, R.A.,Carr, M.D.,TB Structural Genomics Consortium (TBSGC) (登録日: 2004-10-25, 公開日: 2005-06-27, 最終更新日: 2024-05-15)

主引用文献

Renshaw, P.S.,Lightbody, K.L.,Veverka, V.,Muskett, F.W.,Kelly, G.,Frenkiel, T.A.,Gordon, S.V.,Hewinson, R.G.,Burke, B.,Norman, J.,Williamson, R.A.,Carr, M.D.
Structure and Function of the Complex Formed by the Tuberculosis Virulence Factors Cfp-10 and Esat-6
Embo J., 24:2491-, 2005

PubMed Abstract: The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6 have recently been shown to play an essential role in tuberculosis pathogenesis. We have determined the solution structure of the tight, 1:1 complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy to demonstrate specific binding of the complex to the surface of macrophage and monocyte cells. A striking feature of the complex is the long flexible arm formed by the C-terminus of CFP-10, which was found to be essential for binding to the surface of cells. The surface features of the CFP-10.ESAT-6 complex, together with observed binding to specific host cells, strongly suggest a key signalling role for the complex, in which binding to cell surface receptors leads to modulation of host cell behaviour to the advantage of the pathogen.

PubMed: 15973432
DOI: 10.1038/SJ.EMBOJ.7600732

実験手法

SOLUTION NMR