Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

1W57

Structure of the Bifunctional IspDF from Campylobacter jejuni containing Zn

1W57 の概要
エントリーDOI10.2210/pdb1w57/pdb
関連するPDBエントリー1W55
分子名称ISPD/ISPF BIFUNCTIONAL ENZYME, ZINC ION, CYTIDINE-5'-MONOPHOSPHATE, ... (5 entities in total)
機能のキーワードbifunctional enzyme, biosynthetic pathway, isoprenoids, bifunctional, nonmevalonate, transferase
由来する生物種CAMPYLOBACTER JEJUNI
タンパク質・核酸の鎖数1
化学式量合計42770.09
構造登録者
Gabrielsen, M.,Bond, C.S.,Hunter, W.N. (登録日: 2004-08-06, 公開日: 2004-10-04, 最終更新日: 2023-12-13)
主引用文献Gabrielsen, M.,Bond, C.S.,Hallyburton, I.,Hecht, S.,Bacher, A.,Eisenreich, W.,Rohdich, F.,Hunter, W.N.
Hexameric Assembly of the Bifunctional Methylerythritol 2,4-Cyclodiphosphate Synthase and Protein-Protein Associations in the Deoxy-Xylulose-Dependent Pathway of Isoprenoid Precursor Biosynthesis
J.Biol.Chem., 279:52753-, 2004
Cited by
PubMed Abstract: The bifunctional methylerythritol 4-phosphate cytidylyltransferase methylerythritol 2,4-cyclodiphosphate synthase (IspDF) is unusual in that it catalyzes nonconsecutive reactions in the 1-deoxy-D-xylulose 5-phosphate (DOXP) pathway of isoprenoid precursor biosynthesis. The crystal structure of IspDF from the bacterial pathogen Campylobacter jejuni reveals an elongated hexamer with D3 symmetry compatible with the dimeric 2C-methyl-D-erythritol-4-phosphate cytidylyltransferase and trimeric 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase monofunctional enzymes. Complex formation of IspDF with 4-diphosphocytidyl-2C-methyl-D-erythritol kinase (IspE), the intervening enzyme activity in the pathway, has been observed in solution for the enzymes from C. jejuni and Agrobacterium tumefaciens. The monofunctional enzymes (2C-methyl-D-erythritol-4-phosphate cytidylyltransferase, IspE, and 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase) involved in the DOXP biosynthetic pathway of Escherichia coli also show physical associations. We propose that complex formation of the three enzymes at the core of the DOXP pathway can produce an assembly localizing 18 catalytic centers for the early stages of isoprenoid biosynthesis.
PubMed: 15466439
DOI: 10.1074/JBC.M408895200
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (3.09 Å)
構造検証レポート
Validation report summary of 1w57
検証レポート(詳細版)ダウンロードをダウンロード

238895

件を2025-07-16に公開中

PDB statisticsPDBj update infoContact PDBjnumon