1VTV

Molecular structure of (M5DC-DG)3: The role of the methyl group on 5-methyl cytosine in stabilizing Z-DNA

Summary for 1VTV

• Entry DOI: 10.2210/pdb1vtv/pdb
• Descriptor: DNA (5'-D(*(CH3)CP*GP*(CH3)CP*GP*(CH3)CP*G)-3') (1 entity in total)
• Functional Keywords: z-dna, double helix, modified, dna
• Total number of polymer chains: 2
• Total formula weight: 3704.57

Authors

Fujii, S.,Wang, A.H.-J.,Van Der Marel, G.A.,Van Boom, J.H.,Rich, A. (deposition date: 1989-01-10, release date: 2011-07-13, Last modification date: 2023-12-27)

Primary citation

Fujii, S.,Wang, A.H.-J.,Van Der Marel, G.A.,Van Boom, J.H.,Rich, A.
Molecular Structure of (m5dC-dG)3: The Role of the Methyl Group on 5-Methyl Cytosine in Stabilizing Z-DNA
Nucleic Acids Res., 10:7879-7892, 1982

PubMed Abstract: The hexamer (m5 dC-dG)3 has been synthesized and its three-dimensional structure determined by a single crystal X-ray diffraction analysis. The structure has been refined to a final R value of 15.6% at 1.3 A resolution. The molecule forms a left-handed Z-DNA helix which is similar to the unmethylated Z-DNA structure. The presence of the methyl group has resulted in slight changes in the twist angle between successive base pairs and modification of some of the interatomic contacts. Methylation of cytosine in the C5 position is associated with a relative destabilization of the B-DNA structure and a stabilization through hydrophobic bonding of the Z-DNA structure.

PubMed: 7155900

Experimental method

X-RAY DIFFRACTION (1.3 Å)