1VTL

CO-CRYSTAL STRUCTURE OF TBP RECOGNIZING THE MINOR GROOVE OF A TATA ELEMENT

Summary for 1VTL

• Entry DOI: 10.2210/pdb1vtl/pdb
• Descriptor: DNA (5'-D(*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*A)-3'), DNA (5'-D(*TP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*C)-3'), TATA BINDING PROTEIN (TBP) (3 entities in total)
• Functional Keywords: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• Biological source: ARABIDOPSIS THALIANA (mouse-ear cress)
• Total number of polymer chains: 6
• Total formula weight: 58948.76

Authors

Kim, J.L.,Nikolov, D.B.,Burley, S.K. (deposition date: 1993-12-17, release date: 2011-07-13, Last modification date: 2023-12-27)

Primary citation

Kim, J.L.,Nikolov, D.B.,Burley, S.K.
Co-Crystal Structure of TBP Recognizing the Minor Groove of a TATA Element
Nature, 365:520-527, 1993

PubMed Abstract: The three-dimensional structure of a TATA-box binding polypeptide complexed with the TATA element of the adenovirus major late promoter has been determined by X-ray crystallography at 2.25 A resolution. Binding of the saddle-shaped protein induces a conformational change in the DNA, inducing sharp kinks at either end of the sequence TATAAAAG. Between the kinks, the right-handed double helix is smoothly curved and partially unwound, presenting a widened minor groove to TBP's concave, antiparallel beta-sheet. Side-chain/base interactions are restricted to the minor groove, and include hydrogen bonds, van der Waals contacts and phenylalanine-base stacking interactions.

PubMed: 8413605
DOI: 10.1038/365520a0

Experimental method

X-RAY DIFFRACTION (2.25 Å)