1VTL

CO-CRYSTAL STRUCTURE OF TBP RECOGNIZING THE MINOR GROOVE OF A TATA ELEMENT

1VTL の概要

• エントリーDOI: 10.2210/pdb1vtl/pdb
• 分子名称: DNA (5'-D(*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*A)-3'), DNA (5'-D(*TP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*C)-3'), TATA BINDING PROTEIN (TBP) (3 entities in total)
• 機能のキーワード: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• 由来する生物種: ARABIDOPSIS THALIANA (mouse-ear cress)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 58948.76

構造登録者

Kim, J.L.,Nikolov, D.B.,Burley, S.K. (登録日: 1993-12-17, 公開日: 2011-07-13, 最終更新日: 2023-12-27)

主引用文献

Kim, J.L.,Nikolov, D.B.,Burley, S.K.
Co-Crystal Structure of TBP Recognizing the Minor Groove of a TATA Element
Nature, 365:520-527, 1993

PubMed Abstract: The three-dimensional structure of a TATA-box binding polypeptide complexed with the TATA element of the adenovirus major late promoter has been determined by X-ray crystallography at 2.25 A resolution. Binding of the saddle-shaped protein induces a conformational change in the DNA, inducing sharp kinks at either end of the sequence TATAAAAG. Between the kinks, the right-handed double helix is smoothly curved and partially unwound, presenting a widened minor groove to TBP's concave, antiparallel beta-sheet. Side-chain/base interactions are restricted to the minor groove, and include hydrogen bonds, van der Waals contacts and phenylalanine-base stacking interactions.

PubMed: 8413605
DOI: 10.1038/365520a0

実験手法

X-RAY DIFFRACTION (2.25 Å)