1VIO

Crystal structure of pseudouridylate synthase

1VIO の概要

• エントリーDOI: 10.2210/pdb1vio/pdb
• 分子名称: Ribosomal small subunit pseudouridine synthase A, 1,4-BUTANEDIOL (3 entities in total)
• 機能のキーワード: structural genomics, lyase
• 由来する生物種: Haemophilus influenzae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55310.13

構造登録者

Structural GenomiX (登録日: 2003-12-01, 公開日: 2003-12-30, 最終更新日: 2023-12-27)

主引用文献

Matte, A.,Louie, G.V.,Sivaraman, J.,Cygler, M.,Burley, S.K.
Structure of the pseudouridine synthase RsuA from Haemophilus influenzae.
Acta Crystallogr.,Sect.F, 61:350-354, 2005

PubMed Abstract: The structure of the pseudouridine synthase RsuA from Haemophilus influenza, which catalyzes the conversion of uridine to pseudouridine at a single position within 16S ribosomal RNA, has been determined at 1.59 A resolution and compared with that of Escherichia coli RsuA. The H. influenza enzyme contains an N-terminal S4-like alpha3beta4 domain followed by a catalytic domain, as observed in the structure of E. coli RsuA. Whereas the individual domains of E. coli and H. influenza RsuA are structurally similar, their relative spatial disposition differs greatly between the two structures. The former displays an extended open conformation with no direct contacts between the domains, while the latter is in a closed conformation with a large interface between the two domains. Domain closure presents several basic and polar residues into a putative RNA-binding cleft. It is proposed that this relative repositioning of the S4 and catalytic domains is used to modulate the shape and size of the rRNA-binding site in RsuA and in other pseudouridine synthases possessing S4 domains.

PubMed: 16511038
DOI: 10.1107/S1744309105005920

実験手法

X-RAY DIFFRACTION (1.59 Å)