1VG1

GDP-Bound Rab7

1VG1 の概要

• エントリーDOI: 10.2210/pdb1vg1/pdb
• 関連するPDBエントリー: 1VG0 1VG8 1VG9
• 分子名称: Ras-related protein Rab-7, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: gtp-binding protein, protein transport
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein ; Cytoplasmic side : P09527
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21588.50

構造登録者

Rak, A.,Pylypenko, O.,Niculae, A.,Pyatkov, K.,Goody, R.S.,Alexandrov, K. (登録日: 2004-04-22, 公開日: 2004-07-20, 最終更新日: 2023-10-25)

主引用文献

Rak, A.,Pylypenko, O.,Niculae, A.,Pyatkov, K.,Goody, R.S.,Alexandrov, K.
Structure of the Rab7:REP-1 complex: insights into the mechanism of Rab prenylation and choroideremia disease
Cell(Cambridge,Mass.), 117:749-760, 2004

PubMed Abstract: Members of the RabGDI/REP family serve as multifunctional regulators of the Rab family of GTP binding proteins. Mutations in members of this family, such as REP-1, lead to abnormalities, including progressive retinal degradation (choroideremia) in humans. The crystal structures of the REP-1 protein in complex with monoprenylated or C-terminally truncated Rab7 proteins revealed that Rab7 interacts with the Rab binding platform of REP-1 via an extended interface involving the Switch 1 and 2 regions. The C terminus of the REP-1 molecule functions as a mobile lid covering a conserved hydrophobic patch on the surface of REP-1 that in the complex coordinates the C terminus of Rab proteins. Using semisynthetic fluorescent Rab27A, we demonstrate that although Rab27A can be prenylated by REP-2, this reaction can be effectively inhibited by other Rab proteins, providing a possible explanation for the accumulation of unprenylated Rab27A in choroideremia.

PubMed: 15186776
DOI: 10.1016/j.cell.2004.05.017

実験手法

X-RAY DIFFRACTION (1.9 Å)