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1VG1

GDP-Bound Rab7

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsENRAF-NONIUS FR591
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2002-07-02
DetectorMARRESEARCH
Spacegroup nameC 2 2 21
Unit cell lengths64.500, 103.200, 51.700
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution18.790 - 1.900
R-factor0.187
Rwork0.187
R-free0.24700
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)Rab7 from REP1:Rab7 complex (PDB code 1VG0)
RMSD bond length0.015
RMSD bond angle1.700
Data scaling softwareXDS
Phasing softwareCNS
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]18.8002.000
High resolution limit [Å]1.9001.900
Number of reflections13877
<I/σ(I)>20.25.7
Completeness [%]99.299
Redundancy5.75.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.229825% PEG 3350, 0.25M Ammonium Phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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