1VG1
GDP-Bound Rab7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-07-02 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.500, 103.200, 51.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.790 - 1.900 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Rab7 from REP1:Rab7 complex (PDB code 1VG0) |
RMSD bond length | 0.015 |
RMSD bond angle | 1.700 |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.800 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 13877 | |
<I/σ(I)> | 20.2 | 5.7 |
Completeness [%] | 99.2 | 99 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 25% PEG 3350, 0.25M Ammonium Phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |