1UYR

Acetyl-CoA Carboxylase Carboxyltransferase Domain in complex with inhibitor Diclofop

Summary for 1UYR

• Entry DOI: 10.2210/pdb1uyr/pdb
• Related: 1OD2 1OD4 1UYS 1UYT 1UYV
• Descriptor: ACETYL-COA CARBOXYLASE, 2-[4-(2,4-DICHLOROPHENOXY)PHENOXY]PROPANOIC ACID (3 entities in total)
• Functional Keywords: carboxylase, carboxyltransferase, herbicide, transferase
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• Cellular location: Cytoplasm: Q00955
• Total number of polymer chains: 2
• Total formula weight: 167543.38

Authors

Zhang, H.,Tweel, B.,Tong, L. (deposition date: 2004-03-02, release date: 2004-03-29, Last modification date: 2024-05-08)

Primary citation

Zhang, H.,Tweel, B.,Tong, L.
Molecular Basis for the Inhibition of the Carboxyltransferase Domain of Acetyl-Coenzyme-A Carboxylase by Haloxyfop and Diclofop
Proc.Natl.Acad.Sci.USA, 101:5910-, 2004

PubMed Abstract: Acetyl-CoA carboxylases (ACCs) are crucial for the metabolism of fatty acids, making these enzymes important targets for the development of therapeutics against obesity, diabetes, and other diseases. The carboxyltransferase (CT) domain of ACC is the site of action of commercial herbicides, such as haloxyfop, diclofop, and sethoxydim. We have determined the crystal structures at up to 2.5-A resolution of the CT domain of yeast ACC in complex with the herbicide haloxyfop or diclofop. The inhibitors are bound in the active site, at the interface of the dimer of the CT domain. Unexpectedly, inhibitor binding requires large conformational changes for several residues in this interface, which create a highly conserved hydrophobic pocket that extends deeply into the core of the dimer. Two residues that affect herbicide sensitivity are located in this binding site, and mutation of these residues disrupts the structure of the domain. Other residues in the binding site are strictly conserved among the CT domains.

PubMed: 15079078
DOI: 10.1073/PNAS.0400891101

Experimental method

X-RAY DIFFRACTION (2.5 Å)