1UW1

A Novel ADP- and Zinc-binding fold from function-directed in vitro evolution

Summary for 1UW1

Entry DOI	10.2210/pdb1uw1/pdb
Descriptor	ARTIFICIAL NUCLEOTIDE BINDING PROTEIN (ANBP), ADENOSINE-5'-DIPHOSPHATE, ZINC ION, ... (4 entities in total)
Functional Keywords	artificial nucleotide binding protein, nucleotide binding protein, in vitro evolution, de novo protein
Biological source	SYNTHETIC CONSTRUCT
Total number of polymer chains	1
Total formula weight	10098.59
Authors	Lo Surdo, P.,Walsh, M.A.,Sollazzo, M. (deposition date: 2004-01-28, release date: 2004-03-26, Last modification date: 2024-05-08)
Primary citation	Lo Surdo, P.,Walsh, M.A.,Sollazzo, M. A Novel Adp- and Zinc-Binding Fold from Function-Directed in Vitro Evolution Nat.Struct.Mol.Biol., 11:382-, 2004 Cited by PubMed Abstract: A great challenge to biologists is to create proteins with novel folds and tailored functions. As an alternative to de novo protein design, we investigated the structure of a randomly generated protein targeted to bind ATP. The crystal structure reveals a novel alpha/beta fold bound to its ligand, representing both the first protein structure derived from in vitro evolution and the first nucleotide-binding protein stabilized by a zinc ion. PubMed: 15024384 DOI: 10.1038/NSMB745 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.94 Å)

Structure validation