1ULC

CGL2 in complex with lactose

1ULC の概要

• エントリーDOI: 10.2210/pdb1ulc/pdb
• 関連するPDBエントリー: 1A3K 1BKZ 1C1F 1GAN 1IS5 1LCL 1QMJ 1SLA 1UL9 1ULD 1ULE 1ULF 1ULG
• 関連するBIRD辞書のPRD_ID: PRD_900004
• 分子名称: galectin-2, beta-D-galactopyranose-(1-4)-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: galectin, lectin, beta-galactoside binding lectin, sugar binding, sugar binding protein
• 由来する生物種: Coprinopsis cinerea
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34218.49

構造登録者

Walser, P.J.,Haebel, P.W.,Kuenzler, M.,Kues, U.,Aebi, M.,Ban, N. (登録日: 2003-09-12, 公開日: 2004-04-20, 最終更新日: 2023-12-27)

主引用文献

Walser, P.J.,Haebel, P.W.,Kuenzler, M.,Sargent, D.,Kues, U.,Aebi, M.,Ban, N.
Structure and Functional Analysis of the Fungal Galectin CGL2
STRUCTURE, 12:689-702, 2004

PubMed Abstract: Recognition of and discrimination between potential glyco-substrates is central to the function of galectins. Here we dissect the fundamental parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes reveal that this prototype galectin achieves increased substrate specificity by accommodating substituted oligosaccharides of the mammalian blood group A/B type in an extended binding cleft. Kinetic studies on wild-type and mutant CGL2 proteins demonstrate that the tetrameric organization is essential for functionality. The geometric constraints due to the orthogonal orientation of the four binding sites have important consequences on substrate binding and selectivity.

PubMed: 15062091
DOI: 10.1016/j.str.2004.03.002

実験手法

X-RAY DIFFRACTION (2.6 Å)