1UCU

R-type straight flagellar filament made of full-length flagellin

Summary for 1UCU

• Entry DOI: 10.2210/pdb1ucu/pdb
• Descriptor: phase 1 Flagellin (1 entity in total)
• Functional Keywords: flagellin, flagellar filament, helical reconstruction, structural protein
• Biological source: Salmonella typhimurium
• Cellular location: Secreted: P06179
• Total number of polymer chains: 1
• Total formula weight: 51551.20

Authors

Yonekura, K.,Maki-Yonekura, S.,Namba, K. (deposition date: 2003-04-22, release date: 2003-08-12, Last modification date: 2024-03-13)

Primary citation

Yonekura, K.,Maki-Yonekura, S.,Namba, K.
Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy
NATURE, 424:643-650, 2003

PubMed Abstract: The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagellin fragment lacking about 100 terminal residues revealed the protofilament structure, but the full filament structure is still essential for understanding the mechanism of supercoiling and polymerization. Here we report a complete atomic model of the R-type filament by electron cryomicroscopy. A density map obtained from image data up to 4 A resolution shows the feature of alpha-helical backbone and some large side chains. The atomic model built on the map reveals intricate molecular packing and an alpha-helical coiled coil formed by the terminal chains in the inner core of the filament, with its intersubunit hydrophobic interactions having an important role in stabilizing the filament.

PubMed: 12904785
DOI: 10.1038/nature01830

Experimental method

ELECTRON MICROSCOPY (4 Å)