1UBD

CO-CRYSTAL STRUCTURE OF HUMAN YY1 ZINC FINGER DOMAIN BOUND TO THE ADENO-ASSOCIATED VIRUS P5 INITIATOR ELEMENT

1UBD の概要

• エントリーDOI: 10.2210/pdb1ubd/pdb
• 分子名称: DNA (5'-D(*AP*GP*GP*GP*TP*CP*TP*CP*CP*AP*TP*TP*TP*TP*GP*AP*A P*GP*CP*G)-3'), DNA (5'-D(*CP*GP*CP*TP*TP*CP*AP*AP*AP*AP*TP*GP*GP*AP*GP*AP*C P*CP*CP*T)-3'), PROTEIN (YY1 ZINC FINGER DOMAIN), ... (5 entities in total)
• 機能のキーワード: transcription initiation, initiator element, yy1, zinc finger protein, dna- protein recognition, complex (transcription regulation-dna), transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 26719.07

構造登録者

Houbaviy, H.B.,Usheva, A.,Shenk, T.,Burley, S.K. (登録日: 1996-10-04, 公開日: 1996-12-23, 最終更新日: 2024-02-14)

主引用文献

Houbaviy, H.B.,Usheva, A.,Shenk, T.,Burley, S.K.
Cocrystal structure of YY1 bound to the adeno-associated virus P5 initiator.
Proc.Natl.Acad.Sci.USA, 93:13577-13582, 1996

PubMed Abstract: Ying-Yang 1 protein (YY1) supports specific, unidirectional initiation of messenger RNA production by RNA polymerase II from two adjacent start sites in the adeno-associated virus P5 promoter, a process which is independent of the TATA box-binding protein (TBP). The 2.5-A resolution YY1-initiator element cocrystal structure reveals four zinc fingers recognizing a YY1-binding consensus sequence. Upstream of the transcription start sites protein-DNA contacts involve both strands and downstream they are virtually restricted to the template strand, permitting access to the active center of RNA polymerase II and ensuring specificity and directionality. The observed pattern of protein-DNA contacts also explains YY1 binding to a preformed transcription bubble, and YY1 binding to a DNA/RNA hybrid analog of the P5 promoter region containing a nascent RNA transcript. A model is proposed for YY1-directed, TBP-independent transcription initiation.

PubMed: 8942976
DOI: 10.1073/pnas.93.24.13577

実験手法

X-RAY DIFFRACTION (2.5 Å)