Structure of aminofluorene adduct paired opposite cytosine at the polymerase active site.

Summary for 1UA1

DescriptorDNA primer strand, DNA template strand with aminofluorene adduct, DNA polymerase I, ... (6 entities in total)
Functional Keywordsdna polymerase i; dna replication; klenow fragment; protein-dna complex; aminofluorene; aromatic amine; dna lesion; translation replication, transferase-dna complex, transferase/dna
Biological sourceGeobacillus stearothermophilus
Total number of polymer chains3
Total molecular weight74199.14
Hsu, G.W.,Kiefer, J.R.,Becherel, O.J.,Fuchs, R.P.P.,Beese, L.S. (deposition date: 2004-08-11, release date: 2004-09-28, Last modification date: 2011-07-13)
Primary citation
Hsu, G.W.,Kiefer, J.R.,Becherel, O.J.,Fuchs, R.P.P.,Beese, L.S.
Observing translesion synthesis of an aromatic amine DNA adduct by a high-fidelity DNA polymerase
J.Biol.Chem., 279:50280-50285, 2004
PubMed: 15385534 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M409224200
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.24960.3%0.8%5.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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