1U3M

NMR structure of the chicken prion protein fragment 128-242

Summary for 1U3M

• Entry DOI: 10.2210/pdb1u3m/pdb
• NMR Information: BMRB: 6269
• Descriptor: prion-like protein (1 entity in total)
• Functional Keywords: prion protein, tse, prp, membrane protein
• Biological source: Gallus gallus (chicken)
• Cellular location: Cell membrane; Lipid-anchor, GPI-anchor: P27177
• Total number of polymer chains: 1
• Total formula weight: 13339.88

Authors

Lysek, D.A.,Calzolai, L.,Guntert, P.,Wuthrich, K. (deposition date: 2004-07-22, release date: 2005-01-04, Last modification date: 2024-10-30)

Primary citation

Calzolai, L.,Lysek, D.A.,Perez, D.R.,Guntert, P.,Wuthrich, K.
Prion protein NMR structures of chickens, turtles, and frogs
Proc.Natl.Acad.Sci.Usa, 102:651-655, 2005

PubMed Abstract: The NMR structures of the recombinant prion proteins from chicken (Gallus gallus; chPrP), the red-eared slider turtle (Trachemys scripta; tPrP), and the African clawed frog (Xenopus laevis; xlPrP) are presented. The amino acid sequences of these prion proteins show approximately 30% identity with mammalian prion proteins. All three species form the same molecular architecture as mammalian PrPC, with a long, flexibly disordered tail attached to the N-terminal end of a globular domain. The globular domain in chPrP and tPrP contains three alpha-helices, one short 3(10)-helix, and a short antiparallel beta-sheet. In xlPrP, the globular domain includes three alpha-helices and a somewhat longer beta-sheet than in the other species. The spatial arrangement of these regular secondary structures coincides closely with that of the globular domain in mammalian prion proteins. Based on the low sequence identity to mammalian PrPs, comparison of chPrP, tPrP, and xlPrP with mammalian PrPC structures is used to identify a set of essential amino acid positions for the preservation of the same PrPC fold in birds, reptiles, amphibians, and mammals. There are additional conserved residues without apparent structural roles, which are of interest for the ongoing search for physiological functions of PrPC in healthy organisms.

PubMed: 15647366
DOI: 10.1073/pnas.0408939102

Experimental method

SOLUTION NMR