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1U3C

Crystal Structure of the PHR domain of Cryptochrome 1 from Arabidopsis thaliana

1U3C の概要
エントリーDOI10.2210/pdb1u3c/pdb
関連するPDBエントリー1u3d
分子名称Cryptochrome 1 apoprotein, MAGNESIUM ION, CHLORIDE ION, ... (7 entities in total)
機能のキーワードphotolyase, amppnp, signaling protein
由来する生物種Arabidopsis thaliana (thale cress)
細胞内の位置Cytoplasm: Q43125
タンパク質・核酸の鎖数1
化学式量合計58807.25
構造登録者
Brautigam, C.A.,Smith, B.S.,Ma, Z.,Palnitkar, M.,Tomchick, D.R.,Machius, M.,Deisenhofer, J. (登録日: 2004-07-21, 公開日: 2004-08-24, 最終更新日: 2024-11-20)
主引用文献Brautigam, C.A.,Smith, B.S.,Ma, Z.,Palnitkar, M.,Tomchick, D.R.,Machius, M.,Deisenhofer, J.
Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thaliana.
Proc.Natl.Acad.Sci.USA, 101:12142-12147, 2004
Cited by
PubMed Abstract: Signals generated by cryptochrome (CRY) blue-light photoreceptors are responsible for a variety of developmental and circadian responses in plants. The CRYs are also identified as circadian blue-light photoreceptors in Drosophila and components of the mammalian circadian clock. These flavoproteins all have an N-terminal domain that is similar to photolyase, and most have an additional C-terminal domain of variable length. We present here the crystal structure of the photolyase-like domain of CRY-1 from Arabidopsis thaliana. The structure reveals a fold that is very similar to photolyase, with a single molecule of FAD noncovalently bound to the protein. The surface features of the protein and the dissimilarity of a surface cavity to that of photolyase account for its lack of DNA-repair activity. Previous in vitro experiments established that the photolyase-like domain of CRY-1 can bind Mg.ATP, and we observe a single molecule of an ATP analog bound in the aforementioned surface cavity, near the bound FAD cofactor. The structure has implications for the signaling mechanism of CRY blue-light photoreceptors.
PubMed: 15299148
DOI: 10.1073/pnas.0404851101
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.6 Å)
構造検証レポート
Validation report summary of 1u3c
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-30に公開中

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