1U04

Crystal structure of full length Argonaute from Pyrococcus furiosus

1U04 の概要

• エントリーDOI: 10.2210/pdb1u04/pdb
• 分子名称: hypothetical protein PF0537 (2 entities in total)
• 機能のキーワード: rnai, silencing, argonaute, piwi, paz, risc, slicer, rnase h, hydrolase-gene regulation complex, hydrolase/gene regulation
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 91441.30

構造登録者

Song, J.J.,Smith, S.K.,Hannon, G.J.,Joshua-Tor, L. (登録日: 2004-07-12, 公開日: 2004-08-17, 最終更新日: 2024-10-30)

主引用文献

Song, J.J.,Smith, S.K.,Hannon, G.J.,Joshua-Tor, L.
Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity
Science, 305:1434-1437, 2004

PubMed Abstract: Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNA interference effector complex RNA-induced silencing complex (RISC). However, the identity of "Slicer," the enzyme that cleaves the messenger RNA (mRNA) as directed by the siRNA, has not been resolved. Here, we report the crystal structure of the Argonaute protein from Pyrococcus furiosus at 2.25 angstrom resolution. The structure reveals a crescent-shaped base made up of the amino-terminal, middle, and PIWI domains. The Piwi Argonaute Zwille (PAZ) domain is held above the base by a "stalk"-like region. The PIWI domain (named for the protein piwi) is similar to ribonuclease H, with a conserved active site aspartate-aspartate-glutamate motif, strongly implicating Argonaute as "Slicer." The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage.

PubMed: 15284453
DOI: 10.1126

実験手法

X-RAY DIFFRACTION (2.25 Å)