1TW7

Wide Open 1.3A Structure of a Multi-drug Resistant HIV-1 Protease Represents a Novel Drug Target

Summary for 1TW7

• Entry DOI: 10.2210/pdb1tw7/pdb
• Related: 1RPI 1RQ9 1RV7
• Descriptor: protease, SODIUM ION (3 entities in total)
• Functional Keywords: hiv protease, aids, polyprotein, hydrolase, aspartyl protease, multi-drug resistance
• Biological source: Human immunodeficiency virus 1
• Total number of polymer chains: 2
• Total formula weight: 21525.20

Authors

Martin, P.,Vickrey, J.F.,Proteasa, G.,Jimenez, Y.L.,Wawrzak, Z.,Winters, M.A.,Merigan, T.C.,Kovari, L.C. (deposition date: 2004-06-30, release date: 2005-07-19, Last modification date: 2023-08-23)

Primary citation

Martin, P.,Vickrey, J.F.,Proteasa, G.,Jimenez, Y.L.,Wawrzak, Z.,Winters, M.A.,Merigan, T.C.,Kovari, L.C.
Wide Open 1.3A Structure of a Multi-drug Resistant HIV-1 Protease Represents a Novel Drug Target
Structure, 13:1887-1895, 2005

PubMed Abstract: This report examines structural changes in a highly mutated, clinical multidrug-resistant HIV-1 protease, and the crystal structure has been solved to 1.3 A resolution in the absence of any inhibitor. This protease variant contains codon mutations at positions 10, 36, 46, 54, 62, 63, 71, 82, 84, and 90 that confer resistance to protease inhibitors. Major differences between the wild-type and the variant include a structural change initiated by the M36V mutation and amplified by additional mutations in the flaps of the protease, resulting in a "wide-open" structure that represents an opening that is 8 A wider than the "open" structure of the wild-type protease. A second structural change is triggered by the L90M mutation that results in reshaping the 23-32 segment. A third key structural change of the protease is due to the mutations from longer to shorter amino acid side chains at positions 82 and 84.

PubMed: 16338417
DOI: 10.1016/j.str.2005.11.005

Experimental method

X-RAY DIFFRACTION (1.3 Å)