1TOZ

NMR structure of the human NOTCH-1 ligand binding region

1TOZ の概要

• エントリーDOI: 10.2210/pdb1toz/pdb
• 関連するPDBエントリー: 1EMO 1HJ7 1LMJ
• 分子名称: Neurogenic locus notch homolog protein 1 (1 entity in total)
• 機能のキーワード: notch, egf, calcium binding, ligand binding, module, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein (By similarity). Notch 1 intracellular domain: Nucleus (By similarity): P46531
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12600.01

構造登録者

Hambleton, S.,Valeyev, N.Y.,Muranyi, A.,Knott, V.,Werner, J.M.,Mcmichael, A.J.,Handford, P.A.,Downing, A.K. (登録日: 2004-06-15, 公開日: 2004-10-12, 最終更新日: 2024-11-20)

主引用文献

Hambleton, S.,Valeyev, N.V.,Muranyi, A.,Knott, V.,Werner, J.M.,McMichael, A.J.,Handford, P.A.,Downing, A.K.
Structural and functional properties of the human notch-1 ligand binding region
STRUCTURE, 12:2173-2183, 2004

PubMed Abstract: We present NMR structural and dynamics analysis of the putative ligand binding region of human Notch-1, comprising EGF-like domains 11-13. Functional integrity of an unglycosylated, recombinant fragment was confirmed by calcium-dependent binding of tetrameric complexes to ligand-expressing cells. EGF modules 11 and 12 adopt a well-defined, rod-like orientation rigidified by calcium. The interdomain tilt is similar to that found in previously studied calcium binding EGF pairs, but the angle of twist is significantly different. This leads to an extended double-stranded beta sheet structure, spanning the two EGF modules. Based on the conservation of residues involved in interdomain hydrophobic packing, we propose this arrangement to be prototypical of a distinct class of EGF linkages. On this premise, we have constructed a model of the 36 EGF modules of the Notch extracellular domain that enables predictions to be made about the general role of calcium binding to this region.

PubMed: 15576031
DOI: 10.1016/j.str.2004.09.012

実験手法

SOLUTION NMR