1TJY

Crystal Structure of Salmonella typhimurium AI-2 receptor LsrB in complex with R-THMF

1TJY の概要

• エントリーDOI: 10.2210/pdb1tjy/pdb
• 関連するPDBエントリー: 1JX6 1TM2
• 分子名称: sugar transport protein, (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (3 entities in total)
• 機能のキーワード: protein-ligand complex, signaling protein
• 由来する生物種: Salmonella typhimurium
• 細胞内の位置: Periplasm : Q8Z2X8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34400.78

構造登録者

Miller, S.T.,Xavier, K.B.,Campagna, S.R.,Taga, M.E.,Semmelhack, M.F.,Bassler, B.L.,Hughson, F.M. (登録日: 2004-06-07, 公開日: 2004-09-28, 最終更新日: 2024-10-30)

主引用文献

Miller, S.T.,Xavier, K.B.,Campagna, S.R.,Taga, M.E.,Semmelhack, M.F.,Bassler, B.L.,Hughson, F.M.
Salmonella typhimurium Recognizes a Chemically Distinct Form of the Bacterial Quorum-Sensing Signal AI-2
Mol.Cell, 15:677-687, 2004

PubMed Abstract: Bacterial populations use cell-cell communication to coordinate community-wide regulation of processes such as biofilm formation, virulence, and bioluminescence. This phenomenon, termed quorum sensing, is mediated by small molecule signals known as autoinducers. While most autoinducers are species specific, autoinducer-2 (AI-2), first identified in the marine bacterium Vibrio harveyi, is produced and detected by many Gram-negative and Gram-positive bacteria. The crystal structure of the V. harveyi AI-2 signaling molecule bound to its receptor protein revealed an unusual furanosyl borate diester. Here, we present the crystal structure of a second AI-2 signal binding protein, LsrB from Salmonella typhimurium. We find that LsrB binds a chemically distinct form of the AI-2 signal, (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF), that lacks boron. Our results demonstrate that two different species of bacteria recognize two different forms of the autoinducer signal, both derived from 4,5-dihydroxy-2,3-pentanedione (DPD), and reveal new sophistication in the chemical lexicon used by bacteria in interspecies signaling.

PubMed: 15350213
DOI: 10.1016/j.molcel.2004.07.020

実験手法

X-RAY DIFFRACTION (1.3 Å)