1JX6

CRYSTAL STRUCTURE OF LUXP FROM VIBRIO HARVEYI COMPLEXED WITH AUTOINDUCER-2

Summary for 1JX6

• Entry DOI: 10.2210/pdb1jx6/pdb
• Descriptor: LUXP PROTEIN, CALCIUM ION, 3A-METHYL-5,6-DIHYDRO-FURO[2,3-D][1,3,2]DIOXABOROLE-2,2,6,6A-TETRAOL, ... (4 entities in total)
• Functional Keywords: protein-ligand complex, signaling protein
• Biological source: Vibrio harveyi
• Cellular location: Periplasm (Probable): P54300
• Total number of polymer chains: 1
• Total formula weight: 39298.75

Authors

Chen, X.,Schauder, S.,Potier, N.,Van Dorsselaer, A.,Pelczer, I.,BassleR, B.L.,Hughson, F.M. (deposition date: 2001-09-05, release date: 2002-02-04, Last modification date: 2024-02-07)

Primary citation

Chen, X.,Schauder, S.,Potier, N.,Van Dorsselaer, A.,Pelczer, I.,Bassler, B.L.,Hughson, F.M.
Structural identification of a bacterial quorum-sensing signal containing boron.
Nature, 415:545-549, 2002

PubMed Abstract: Cell-cell communication in bacteria is accomplished through the exchange of extracellular signalling molecules called autoinducers. This process, termed quorum sensing, allows bacterial populations to coordinate gene expression. Community cooperation probably enhances the effectiveness of processes such as bioluminescence, virulence factor expression, antibiotic production and biofilm development. Unlike other autoinducers, which are specific to a particular species of bacteria, a recently discovered autoinducer (AI-2) is produced by a large number of bacterial species. AI-2 has been proposed to serve as a 'universal' signal for inter-species communication. The chemical identity of AI-2 has, however, proved elusive. Here we present the crystal structure of an AI-2 sensor protein, LuxP, in a complex with autoinducer. The bound ligand is a furanosyl borate diester that bears no resemblance to previously characterized autoinducers. Our findings suggest that addition of naturally occurring borate to an AI-2 precursor generates active AI-2. Furthermore, they indicate a potential biological role for boron, an element required by a number of organisms but for unknown reasons.

PubMed: 11823863
DOI: 10.1038/415545a

Experimental method

X-RAY DIFFRACTION (1.5 Å)