1TJY
Crystal Structure of Salmonella typhimurium AI-2 receptor LsrB in complex with R-THMF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.824, 76.623, 109.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.250 - 1.300 |
| R-factor | 0.15643 |
| Rwork | 0.156 |
| R-free | 0.17156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.257 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.250 | 1.334 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 77033 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 97.0 | 87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K |
