1T19
Early intermediate IE2 from time-resolved crystallography of the E46Q mutant of PYP
Summary for 1T19
Entry DOI | 10.2210/pdb1t19/pdb |
Related | 1T18 1T1A 1T1B 1T1C |
Descriptor | Photoactive yellow protein, 4'-HYDROXYCINNAMIC ACID (2 entities in total) |
Functional Keywords | photoactive yellow protein, pas domain, photoreceptor |
Biological source | Halorhodospira halophila |
Total number of polymer chains | 1 |
Total formula weight | 14051.75 |
Authors | Rajagopal, S.,Anderson, S.,Srajer, V.,Schmidt, M.,Pahl, R.,Moffat, K. (deposition date: 2004-04-15, release date: 2005-01-18, Last modification date: 2021-10-27) |
Primary citation | Rajagopal, S.,Anderson, S.,Srajer, V.,Schmidt, M.,Pahl, R.,Moffat, K. A Structural Pathway for Signaling in the E46Q Mutant of Photoactive Yellow Protein Structure, 13:55-63, 2005 Cited by PubMed Abstract: In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore. PubMed: 15642261DOI: 10.1016/j.str.2004.10.016 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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