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1T19

Early intermediate IE2 from time-resolved crystallography of the E46Q mutant of PYP

Summary for 1T19
Entry DOI10.2210/pdb1t19/pdb
Related1T18 1T1A 1T1B 1T1C
DescriptorPhotoactive yellow protein, 4'-HYDROXYCINNAMIC ACID (2 entities in total)
Functional Keywordsphotoactive yellow protein, pas domain, photoreceptor
Biological sourceHalorhodospira halophila
Total number of polymer chains1
Total formula weight14051.75
Authors
Rajagopal, S.,Anderson, S.,Srajer, V.,Schmidt, M.,Pahl, R.,Moffat, K. (deposition date: 2004-04-15, release date: 2005-01-18, Last modification date: 2021-10-27)
Primary citationRajagopal, S.,Anderson, S.,Srajer, V.,Schmidt, M.,Pahl, R.,Moffat, K.
A Structural Pathway for Signaling in the E46Q Mutant of Photoactive Yellow Protein
Structure, 13:55-63, 2005
Cited by
PubMed Abstract: In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
PubMed: 15642261
DOI: 10.1016/j.str.2004.10.016
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.6 Å)
Structure validation

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