1SUM

Crystal structure of a hypothetical protein at 2.0 A resolution

Summary for 1SUM

• Entry DOI: 10.2210/pdb1sum/pdb
• Descriptor: Phosphate transport system protein phoU homolog 2, FE (III) ION, NICKEL (II) ION, ... (6 entities in total)
• Functional Keywords: phou, abc transport, pst, structural genomics, berkeley structural genomics center, bsgc, structure funded by nih, protein structure initiative, psi, transport protein
• Biological source: Thermotoga maritima
• Cellular location: Cytoplasm : Q9X256
• Total number of polymer chains: 1
• Total formula weight: 27498.82

Authors

Liu, J.,Lou, Y.,Yokota, H.,Adams, P.D.,Kim, R.,Kim, S.H.,Berkeley Structural Genomics Center (BSGC) (deposition date: 2004-03-26, release date: 2004-08-24, Last modification date: 2024-11-13)

Primary citation

Liu, J.,Lou, Y.,Yokota, H.,Adams, P.D.,Kim, R.,Kim, S.H.
Crystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters.
J.Biol.Chem., 280:15960-15966, 2005

PubMed Abstract: PhoU proteins are known to play a role in the regulation of phosphate uptake. In Thermotoga maritima, two PhoU homologues have been identified bioinformatically. Here we report the crystal structure of one of the PhoU homologues at 2.0 A resolution. The structure of the PhoU protein homologue contains a highly symmetric new structural fold composed of two repeats of a three-helix bundle. The structure unexpectedly revealed a trinuclear and a tetranuclear iron cluster that were found to be bound on the surface. Each of the two multinuclear iron clusters is coordinated by a conserved E(D)XXXD motif pair. Our structure reveals a new class of metalloprotein containing multinuclear iron clusters. The possible functional implication based on the structure are discussed.

PubMed: 15716271
DOI: 10.1074/jbc.M414117200

Experimental method

X-RAY DIFFRACTION (2 Å)