1SU3

X-ray structure of human proMMP-1: New insights into collagenase action

1SU3 の概要

• エントリーDOI: 10.2210/pdb1su3/pdb
• 分子名称: Interstitial collagenase, CALCIUM ION, CHLORIDE ION, ... (8 entities in total)
• 機能のキーワード: prodomain, hemopexin domain, exocite, structural proteomics in europe, spine, structural genomics, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted, extracellular space, extracellular matrix (Probable): P03956
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 105241.96

構造登録者

Jozic, D.,Bourenkov, G.,Lim, N.H.,Nagase, H.,Bode, W.,Maskos, K.,Structural Proteomics in Europe (SPINE) (登録日: 2004-03-26, 公開日: 2004-12-21, 最終更新日: 2024-10-09)

主引用文献

Jozic, D.,Bourenkov, G.,Lim, N.H.,Visse, R.,Nagase, H.,Bode, W.,Maskos, K.
X-ray structure of human proMMP-1: new insights into procollagenase activation and collagen binding.
J.Biol.Chem., 280:9578-9585, 2005

PubMed Abstract: Vertebrate collagenases, members of the matrix metalloproteinase (MMP) family, initiate interstitial fibrillar collagen breakdown. It is essential in many biological processes, and unbalanced collagenolysis is associated with diseases such as arthritis, cancer, atherosclerosis, aneurysm, and fibrosis. These metalloproteinases are secreted from the cell as inactive precursors, procollagenases (proMMPs). To gain insights into the structural basis of their activation mechanisms and collagen binding, we have crystallized recombinant human proMMP-1 and determined its structure to 2.2 A resolution. The catalytic metalloproteinase domain and the C-terminal hemopexin (Hpx) domain show the classical MMP-fold, but the structure has revealed new features in surface loops and domain interaction. The prodomain is formed by a three-helix bundle and gives insight into the stepwise activation mechanism of proMMP-1. The prodomain interacts with the Hpx domain, which affects the position of the Hpx domain relative to the catalytic domain. This interaction results in a "closed" configuration of proMMP-1 in contrast to the "open" configuration observed previously for the structure of active MMP-1. This is the first evidence of mobility of the Hpx domain in relation to the catalytic domain, providing an important clue toward the understanding of the collagenase-collagen interaction and subsequent collagenolysis.

PubMed: 15611040
DOI: 10.1074/jbc.M411084200

実験手法

X-RAY DIFFRACTION (2.2 Å)