1SPB

SUBTILISIN BPN' PROSEGMENT (77 RESIDUES) COMPLEXED WITH A MUTANT SUBTILISIN BPN' (266 RESIDUES). CRYSTAL PH 4.6. CRYSTALLIZATION TEMPERATURE 20 C DIFFRACTION TEMPERATURE-160 C

1SPB の概要

• エントリーDOI: 10.2210/pdb1spb/pdb
• 分子名称: SUBTILISIN BPN' PROSEGMENT, SUBTILISIN BPN', SODIUM ION, ... (4 entities in total)
• 機能のキーワード: propeptide, foldase, folding catalyst, activation domain, complex (serine proteinase-prosegment) complex, complex (serine proteinase/prosegment)
• 由来する生物種: Bacillus amyloliquefaciens; 詳細
• 細胞内の位置: Secreted: P00782 P00782
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35167.20

構造登録者

Gallagher, D.T.,Gilliland, G.L.,Wang, L.,Bryan, P.N. (登録日: 1995-06-21, 公開日: 1995-10-15, 最終更新日: 2024-02-14)

主引用文献

Gallagher, T.,Gilliland, G.,Wang, L.,Bryan, P.
The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'.
Structure, 3:907-914, 1995

PubMed Abstract: The folding of the bacterial protease subtilisin BPN' (SBT) is dependent on its 77-residue prosegment, which is then autocatalytically removed to give the mature enzyme. Mature subtilisin represents a class of proteins that lacks an efficient folding pathway. Refolding of mature SBT is extremely slow unless catalyzed by the independently expressed prosegment, leading to a bimolecular complex.

PubMed: 8535784
DOI: 10.1016/S0969-2126(01)00225-8

実験手法

X-RAY DIFFRACTION (2 Å)