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1SOZ

Crystal Structure of DegS protease in complex with an activating peptide

1SOZ の概要
エントリーDOI10.2210/pdb1soz/pdb
関連するPDBエントリー1SOT 1VCW
分子名称Protease degS, activating peptide (3 entities in total)
機能のキーワードstress response, protein quality control, pdz, upr, htra, hydrolase
由来する生物種Escherichia coli
細胞内の位置Periplasm (Potential): P31137
タンパク質・核酸の鎖数5
化学式量合計102181.74
構造登録者
Wilken, C.,Kitzing, K.,Kurzbauer, R.,Ehrmann, M.,Clausen, T. (登録日: 2004-03-16, 公開日: 2004-06-08, 最終更新日: 2024-04-03)
主引用文献Wilken, C.,Kitzing, K.,Kurzbauer, R.,Ehrmann, M.,Clausen, T.
Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease
Cell(Cambridge,Mass.), 117:483-494, 2004
Cited by
PubMed Abstract: Gram-negative bacteria respond to misfolded proteins in the cell envelope with the sigmaE-driven expression of periplasmic proteases/chaperones. Activation of sigmaE is controlled by a proteolytic cascade that is initiated by the DegS protease. DegS senses misfolded protein in the periplasm, undergoes autoactivation, and cleaves the antisigma factor RseA. Here, we present the crystal structures of three distinct states of DegS from E. coli. DegS alone exists in a catalytically inactive form. Binding of stress-signaling peptides to its PDZ domain induces a series of conformational changes that activates protease function. Backsoaking of crystals containing the DegS-activator complex revealed the presence of an active/inactive hybrid structure and demonstrated the reversibility of activation. Taken together, the structural data illustrate in molecular detail how DegS acts as a periplasmic stress sensor. Our results suggest a novel regulatory role for PDZ domains and unveil a novel mechanism of reversible protease activation.
PubMed: 15137941
DOI: 10.1016/S0092-8674(04)00454-4
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.4 Å)
構造検証レポート
Validation report summary of 1soz
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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