1SOR

Aquaporin-0 membrane junctions reveal the structure of a closed water pore

1SOR の概要

• エントリーDOI: 10.2210/pdb1sor/pdb
• 分子名称: Aquaporin-0 (1 entity in total)
• 機能のキーワード: membrane junction, water channel, membrane protein
• 由来する生物種: Ovis aries (sheep)
• 細胞内の位置: Cell membrane ; Multi- pass membrane protein : Q6J8I9
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25173.44

構造登録者

Gonen, T.,Sliz, P.,Kistler, J.,Cheng, Y.,Walz, T. (登録日: 2004-03-15, 公開日: 2004-05-11, 最終更新日: 2023-08-23)

主引用文献

Gonen, T.,Sliz, P.,Kistler, J.,Cheng, Y.,Walz, T.
Aquaporin-0 membrane junctions reveal the structure of a closed water pore
Nature, 429:193-197, 2004

PubMed Abstract: The lens-specific water pore aquaporin-0 (AQP0) is the only aquaporin known to form membrane junctions in vivo. We show here that AQP0 from the lens core, containing some carboxy-terminally cleaved AQP0, forms double-layered crystals that recapitulate in vivo junctions. We present the structure of the AQP0 membrane junction as determined by electron crystallography. The junction is formed by three localized interactions between AQP0 molecules in adjoining membranes, mainly mediated by proline residues conserved in AQP0s from different species but not present in most other aquaporins. Whereas all previously determined aquaporin structures show the pore in an open conformation, the water pore is closed in AQP0 junctions. The water pathway in AQP0 also contains an additional pore constriction, not seen in other known aquaporin structures, which may be responsible for pore gating.

PubMed: 15141214
DOI: 10.1038/nature02503

実験手法

ELECTRON CRYSTALLOGRAPHY (3 Å)