1SON

ADENYLOSUCCINATE SYNTHETASE IN COMPLEX WITH THE NATURAL FEEDBACK INHIBITOR AMP

1SON の概要

• エントリーDOI: 10.2210/pdb1son/pdb
• 分子名称: ADENYLOSUCCINATE SYNTHETASE, ADENOSINE MONOPHOSPHATE, BETA-MERCAPTOETHANOL, ... (4 entities in total)
• 機能のキーワード: purine nucleotide biosynthesis, gtp-hydrolyzing enzyme, herbicide, ligase, synthetase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A7D4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47694.95

構造登録者

Cowan-Jacob, S.W. (登録日: 1996-05-07, 公開日: 1997-09-04, 最終更新日: 2023-08-09)

主引用文献

Fonne-Pfister, R.,Chemla, P.,Ward, E.,Girardet, M.,Kreuz, K.E.,Honzatko, R.B.,Fromm, H.J.,Schar, H.P.,Grutter, M.G.,Cowan-Jacob, S.W.
The mode of action and the structure of a herbicide in complex with its target: binding of activated hydantocidin to the feedback regulation site of adenylosuccinate synthetase.
Proc.Natl.Acad.Sci.USA, 93:9431-9436, 1996

PubMed Abstract: (+)-Hydantocidin, a recently discovered natural spironucleoside with potent herbicidal activity, is shown to be a proherbicide that, after phosphorylation at the 5' position, inhibits adenylosuccinate synthetase, an enzyme involved in de novo purine synthesis. The mode of binding of hydantocidin 5'-monophosphate to the target enzyme was analyzed by determining the crystal structure of the enzyme-inhibitor complex at 2.6-A resolution. It was found that adenylosuccinate synthetase binds the phosphorylated compound in the same fashion as it does adenosine 5'-monophosphate, the natural feedback regulator of this enzyme. This work provides the first crystal structure of a herbicide-target complex reported to date.

PubMed: 8790347
DOI: 10.1073/pnas.93.18.9431

実験手法

X-RAY DIFFRACTION (2.55 Å)