1SNR

Nitric oxide bound to Cu nitrite reductase

Summary for 1SNR

• Entry DOI: 10.2210/pdb1snr/pdb
• Descriptor: Copper-containing nitrite reductase, COPPER (I) ION, COPPER (II) ION, ... (7 entities in total)
• Functional Keywords: nitric oxide, nitrite reductase, oxidoreductase
• Biological source: Alcaligenes faecalis
• Cellular location: Periplasm: P38501
• Total number of polymer chains: 3
• Total formula weight: 111701.73

Authors

Tocheva, E.I.,Rosell, F.I.,Mauk, A.G.,Murphy, M.E. (deposition date: 2004-03-11, release date: 2004-06-22, Last modification date: 2024-02-14)

Primary citation

Tocheva, E.I.,Rosell, F.I.,Mauk, A.G.,Murphy, M.E.
Side-on copper-nitrosyl coordination by nitrite reductase.
Science, 304:867-870, 2004

PubMed Abstract: A copper-nitrosyl intermediate forms during the catalytic cycle of nitrite reductase, the enzyme that mediates the committed step in bacterial denitrification. The crystal structure of a type 2 copper-nitrosyl complex of nitrite reductase reveals an unprecedented side-on binding mode in which the nitrogen and oxygen atoms are nearly equidistant from the copper cofactor. Comparison of this structure with a refined nitrite-bound crystal structure explains how coordination can change between copper-oxygen and copper-nitrogen during catalysis. The side-on copper-nitrosyl in nitrite reductase expands the possibilities for nitric oxide interactions in copper proteins such as superoxide dismutase and prions.

PubMed: 15131305
DOI: 10.1126/science.1095109

Experimental method

X-RAY DIFFRACTION (1.31 Å)