Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

1SJ8

Solution Structure of the R1R2 Domains of Talin

Summary for 1SJ8
Entry DOI10.2210/pdb1sj8/pdb
Related1SJ7
DescriptorTalin 1 (2 entities in total)
Functional Keywordsstructural protein
Biological sourceMus musculus (house mouse)
Cellular locationCell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P26039
Total number of polymer chains1
Total formula weight31512.32
Authors
Papagrigoriou, E.,Gingras, A.R.,Barsukov, I.L.,Critchley, D.R.,Emsley, J. (deposition date: 2004-03-03, release date: 2004-08-24, Last modification date: 2023-08-23)
Primary citationPapagrigoriou, E.,Gingras, A.R.,Barsukov, I.L.,Bate, N.,Fillingham, I.J.,Patel, B.,Frank, R.,Ziegler, W.H.,Roberts, G.C.,Critchley, D.R.,Emsley, J.
Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle
EMBO J., 23:2942-2951, 2004
Cited by
PubMed Abstract: The interaction between the cytoskeletal proteins talin and vinculin plays a key role in integrin-mediated cell adhesion and migration. We have determined the crystal structures of two domains from the talin rod spanning residues 482-789. Talin 482-655, which contains a vinculin-binding site (VBS), folds into a five-helix bundle whereas talin 656-789 is a four-helix bundle. We show that the VBS is composed of a hydrophobic surface spanning five turns of helix 4. All the key side chains from the VBS are buried and contribute to the hydrophobic core of the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix bundle represents an inactive conformation, and mutations that disrupt the hydrophobic core or deletion of helix 5 are required to induce an active conformation in which the VBS is exposed. We also report the crystal structure of the N-terminal vinculin head domain in complex with an activated form of talin. Activation of the VBS in talin and the recruitment of vinculin may support the maturation of small integrin/talin complexes into more stable adhesions.
PubMed: 15272303
DOI: 10.1038/sj.emboj.7600285
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.6 Å)
Structure validation

238582

PDB entries from 2025-07-09

PDB statisticsPDBj update infoContact PDBjnumon