1SHZ

Crystal Structure of the p115RhoGEF rgRGS Domain in A Complex with Galpha(13):Galpha(i1) Chimera

1SHZ の概要

• エントリーDOI: 10.2210/pdb1shz/pdb
• 関連するPDBエントリー: 1AGR 1AZS 1FQJ 1IAP
• 分子名称: Guanine Nucleotide-Binding Protein Galpha(13):Galpha(i1) Chimera, Rho guanine nucleotide exchange factor 1, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: signal transduction, protein complex, signaling protein
• 由来する生物種: Rattus norvegicus (house mouse, Norway rat); 詳細
• 細胞内の位置: Nucleus: P10824; Cytoplasm : Q92888
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 133115.10

構造登録者

Chen, Z.,Singer, W.D.,Sternweis, P.C.,Sprang, S.R. (登録日: 2004-02-26, 公開日: 2005-01-18, 最終更新日: 2023-08-23)

主引用文献

Chen, Z.,Singer, W.D.,Sternweis, P.C.,Sprang, S.R.
Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator.
Nat.Struct.Mol.Biol., 12:191-197, 2005

PubMed Abstract: p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric Galpha subunits. The GAP function of p115RhoGEF resides within the N-terminal region of p115RhoGEF (the rgRGS domain), which includes a module that is structurally similar to RGS (regulators of G-protein signaling) domains. We present here the crystal structure of the rgRGS domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1. Two distinct surfaces of rgRGS interact with Galpha. The N-terminal betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate contacts with the catalytic site of Galpha. The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13.

PubMed: 15665872
DOI: 10.1038/nsmb888

実験手法

X-RAY DIFFRACTION (2.85 Å)