1SHW

EphB2 / EphrinA5 Complex Structure

1SHW の概要

• エントリーDOI: 10.2210/pdb1shw/pdb
• 関連するPDBエントリー: 1NUK 1SHX
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: Ephrin-A5, Ephrin type-B receptor 2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: receptor tyrosine kinase, ephrin signaling, hormone-growth factor-receptor complex, hormone/growth factor/receptor
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37897.90

構造登録者

Himanen, J.P.,Chumley, M.J.,Lackmann, M.,Li, C.,Barton, W.A.,Jeffrey, P.D.,Vearing, C.,Geleick, D.,Feldheim, D.A.,Boyd, A.W. (登録日: 2004-02-26, 公開日: 2004-05-18, 最終更新日: 2024-10-30)

主引用文献

Himanen, J.P.,Chumley, M.J.,Lackmann, M.,Li, C.,Barton, W.A.,Jeffrey, P.D.,Vearing, C.,Geleick, D.,Feldheim, D.A.,Boyd, A.W.,Henkemeyer, M.,Nikolov, D.B.
Repelling class discrimination: ephrin-A5 binds to and activates EphB2 receptor signaling
Nat.Neurosci., 7:501-509, 2004

PubMed Abstract: The interactions between Eph receptor tyrosine kinases and their ephrin ligands regulate cell migration and axon pathfinding. The EphA receptors are generally thought to become activated by ephrin-A ligands, whereas the EphB receptors interact with ephrin-B ligands. Here we show that two of the most widely studied of these molecules, EphB2 and ephrin-A5, which have never been described to interact with each other, do in fact bind one another with high affinity. Exposure of EphB2-expressing cells to ephrin-A5 leads to receptor clustering, autophosphorylation and initiation of downstream signaling. Ephrin-A5 induces EphB2-mediated growth cone collapse and neurite retraction in a model system. We further show, using X-ray crystallography, that the ephrin-A5-EphB2 complex is a heterodimer and is architecturally distinct from the tetrameric EphB2-ephrin-B2 structure. The structural data reveal the molecular basis for EphB2-ephrin-A5 signaling and provide a framework for understanding the complexities of functional interactions and crosstalk between A- and B-subclass Eph receptors and ephrins.

PubMed: 15107857
DOI: 10.1038/nn1237

実験手法

X-RAY DIFFRACTION (2.2 Å)