1SDO

Crystal Structure of Restriction Endonuclease BstYI

1SDO の概要

• エントリーDOI: 10.2210/pdb1sdo/pdb
• 分子名称: BstYI (2 entities in total)
• 機能のキーワード: restriction endonuclease, hydrolase
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23222.67

構造登録者

Townson, S.A.,Samuelson, J.C.,Vanamee, E.S.,Edwards, T.A.,Escalante, C.R.,Xu, S.Y.,Aggarwal, A.K. (登録日: 2004-02-13, 公開日: 2004-05-11, 最終更新日: 2024-02-14)

主引用文献

Townson, S.A.,Samuelson, J.C.,Vanamee, E.S.,Edwards, T.A.,Escalante, C.R.,Xu, S.Y.,Aggarwal, A.K.
Crystal Structure of BstYI at 1.85 A Resolution: A Thermophilic Restriction Endonuclease with Overlapping Specificities to BamHI and BglII
J.Mol.Biol., 338:725-733, 2004

PubMed Abstract: We report here the structure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence specificities to BamHI and BglII. BstYI, a thermophilic endonuclease, recognizes and cleaves the degenerate hexanucleotide sequence 5'-RGATCY-3' (where R=A or G and Y=C or T), cleaving DNA after the 5'-R on each strand to produce four-base (5') staggered ends. The crystal structure of free BstYI was solved at 1.85A resolution by multi-wavelength anomalous dispersion (MAD) phasing. Comparison with BamHI and BglII reveals a strong structural consensus between all three enzymes mapping to the alpha/beta core domain and residues involved in catalysis. Unexpectedly, BstYI also contains an additional "arm" substructure outside of the core protein, which enables the enzyme to adopt a more compact, intertwined dimer structure compared with BamHI and BglII. This arm substructure may underlie the thermostability of BstYI. We identify putative DNA recognition residues and speculate as to how this enzyme achieves a "relaxed" DNA specificity.

PubMed: 15099740
DOI: 10.1016/j.jmb.2004.02.074

実験手法

X-RAY DIFFRACTION (1.85 Å)