1SDO
Crystal Structure of Restriction Endonuclease BstYI
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-01 |
Detector | SBC-2 |
Wavelength(s) | 0.973, 0.9282 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 69.890, 76.610, 78.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.850 |
Rwork | 0.220 |
R-free | 0.24100 |
Structure solution method | MAD |
RMSD bond angle | 0.007 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.850 |
Rmerge | 0.092 |
Number of reflections | 33604 |
<I/σ(I)> | 17.4 |
Completeness [%] | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 30% PEG-400, 0.1M HEPES, 200mM Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |