1SAU

The Gamma subunit of the dissimilatory sulfite reductase (DsrC) from Archaeoglobus fulgidus at 1.1 A resolution

1SAU の概要

• エントリーDOI: 10.2210/pdb1sau/pdb
• 分子名称: sulfite reductase, desulfoviridin-type subunit gamma (2 entities in total)
• 機能のキーワード: orthogonal helical bundle, oxidoreductase
• 由来する生物種: Archaeoglobus fulgidus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13498.66

構造登録者

Mander, G.J.,Weiss, M.S.,Hedderich, R.,Kahnt, J.,Ermler, U.,Warkentin, E. (登録日: 2004-02-09, 公開日: 2005-02-15, 最終更新日: 2024-11-13)

主引用文献

Weiss, M.S.,Mander, G.,Hedderich, R.,Diederichs, K.,Ermler, U.,Warkentin, E.
Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing.
Acta Crystallogr.,Sect.D, 60:686-695, 2004

PubMed Abstract: The structure of the 115 amino-acid residue protein DsvC was determined based on the anomalous scattering provided by the five S atoms present in the structure. By collecting the diffraction data at a wavelength of 1.9 A, the anomalous signal provided by the S atoms was enhanced. However, significant radiation damage occurred during the course of the experiment, which led to differences between different parts of the data set. Only by dividing the total data set into five data sets was it possible to obtain phases; these could then be successfully extended to allow structure determination by the automated model-building program ARP/wARP. A computational correction for the radiation damage was found to significantly improve the success rate in determining the heavy-atom substructure and to improve phasing and refinement statistics.

PubMed: 15039557
DOI: 10.1107/S0907444904003002

実験手法

X-RAY DIFFRACTION (1.12 Å)