1SAU

The Gamma subunit of the dissimilatory sulfite reductase (DsrC) from Archaeoglobus fulgidus at 1.1 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-11
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	39.935, 46.528, 57.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.120
R-factor	0.108 *
R-free	0.13700
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	0.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000 *	1.180
High resolution limit [Å]	1.120 *	1.120 *
Rmerge	0.093 *	0.160 *
Total number of observations	205663 *
Number of reflections	40862 *
<I/σ(I)>	21	5.5
Completeness [%]	93.1	73.5 *
Redundancy	5.0 *	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5	277	TRIS, MPD, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 9.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	58 (%(v/v))
2	1	reservoir	Tris-HCl	0.1 (M)	pH9.5