1S9S
SOLUTION STRUCTURE OF MLV PSI SITE
Summary for 1S9S
| Entry DOI | 10.2210/pdb1s9s/pdb |
| Descriptor | MLV Psi encapsidation site (1 entity in total) |
| Functional Keywords | mlv, a-minor k-turn, stem loop, bulge, g-u mismatch, g-a mismatch, u-u mismatch, a-c mismatch, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 32703.43 |
| Authors | D'Souza, V.,Dey, A.,Habib, D.,Summers, M.F. (deposition date: 2004-02-05, release date: 2004-04-06, Last modification date: 2024-05-22) |
| Primary citation | D'Souza, V.,Dey, A.,Habib, D.,Summers, M.F. NMR structure of the 101-nucleotide core encapsidation signal of the Moloney Murine Leukemia Virus. J.Mol.Biol., 337:427-442, 2004 Cited by PubMed Abstract: The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses. PubMed: 15003457DOI: 10.1016/j.jmb.2004.01.037 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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