1S9H

Crystal Structure of Adeno-associated virus Type 2 Rep40

Summary for 1S9H

• Entry DOI: 10.2210/pdb1s9h/pdb
• Descriptor: Rep 40 protein (2 entities in total)
• Functional Keywords: helicase, aaa+ protein, p-loop, walker a, walker b, sensor 1, replication
• Biological source: Adeno-associated virus - 2
• Total number of polymer chains: 3
• Total formula weight: 90594.63

Authors

James, J.A.,Escalante, C.R.,Yoon-Robarts, M.,Edwards, T.A.,Linden, R.M.,Aggarwal, A.K. (deposition date: 2004-02-04, release date: 2004-05-25, Last modification date: 2024-02-14)

Primary citation

James, J.A.,Escalante, C.R.,Yoon-Robarts, M.,Edwards, T.A.,Linden, R.M.,Aggarwal, A.K.
Crystal Structure of the SF3 Helicase from Adeno-Associated Virus Type 2
Structure, 11:1025-1035, 2003

PubMed Abstract: We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as opposed to bimodular. Altogether, the structural connection to AAA(+) proteins defines the general architecture of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for understanding the role of Rep proteins during AAV DNA replication, packaging, and site-specific integration.

PubMed: 12906833
DOI: 10.1016/S0969-2126(03)00152-7

Experimental method

X-RAY DIFFRACTION (2.4 Å)