1S6Z

Enhanced Green Fluorescent Protein Containing the Y66L Substitution

Summary for 1S6Z

Related1EMG 1RM9 1RMO 1RMP 1QYF
Descriptorgreen fluorescent protein, CHLORIDE ION (3 entities in total)
Functional Keywordschromophore, eleven-stranded beta barrel, trapped intermediate, backbone cyclization, gfp-like protein, luminescent protein
Biological sourcesynthetic construct
Total number of polymer chains1
Total molecular weight26898.82
Authors
Rosenow, M.A.,Huffman, H.A.,Phail, M.E.,Wachter, R.M. (deposition date: 2004-01-28, release date: 2004-05-04, Last modification date: 2018-04-04)
Primary citation
Rosenow, M.A.,Huffman, H.A.,Phail, M.E.,Wachter, R.M.
The Crystal Structure of the Y66L Variant of Green Fluorescent Protein Supports a Cyclization-Oxidation-Dehydration Mechanism for Chromophore Maturation(,).
Biochemistry, 43:4464-4472, 2004
PubMed: 15078092 (PDB entries with the same primary citation)
DOI: 10.1021/bi0361315
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.5 Å)
NMR Information
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.227120.5%2.5%6.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 1s6z
no rotation
Molmil generated image of 1s6z
rotated about x axis by 90°
Molmil generated image of 1s6z
rotated about y axis by 90°