1S0U
eIF2gamma apo
Summary for 1S0U
| Entry DOI | 10.2210/pdb1s0u/pdb |
| Descriptor | Translation initiation factor 2 gamma subunit, ZINC ION (3 entities in total) |
| Functional Keywords | translation initiation, gtpase, ef-1a, trna, eif2, translation |
| Biological source | Methanocaldococcus jannaschii |
| Total number of polymer chains | 1 |
| Total formula weight | 44216.46 |
| Authors | Roll-Mecak, A.,Alone, P.,Cao, C.,Dever, T.E.,Burley, S.K. (deposition date: 2004-01-04, release date: 2004-01-20, Last modification date: 2024-10-16) |
| Primary citation | Roll-Mecak, A.,Alone, P.,Cao, C.,Dever, T.E.,Burley, S.K. X-ray Structure of Translation Initiation Factor eIF2gamma: IMPLICATIONS FOR tRNA AND eIF2alpha BINDING. J.Biol.Chem., 279:10634-10642, 2004 Cited by PubMed Abstract: The x-ray structure of the gamma-subunit of the heterotrimeric translation initiation factor eIF2 has been determined to 2.4-A resolution. eIF2 is a GTPase that delivers the initiator Met-tRNA to the P site on the small ribosomal subunit during a rate-limiting initiation step in translation. The structure of eIF2gamma closely resembles that of EF1A.GTP, consisting of an N-terminal G domain followed by two beta-barrels arranged in a closed configuration with domain II packed against the G domain in the vicinity of the Switch regions. The G domain of eIF2gamma has an unusual zinc ribbon motif, not previously found in other GTPases. Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of eIF2gamma that bind the alpha-subunit and Met-tRNA(i)(Met), respectively. These structural, biochemical, and genetic results provide new insights into eIF2 ternary complex assembly. PubMed: 14688270DOI: 10.1074/jbc.M310418200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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