1RYU

Solution Structure of the SWI1 ARID

Summary for 1RYU

• Entry DOI: 10.2210/pdb1ryu/pdb
• NMR Information: BMRB: 5748
• Descriptor: SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1 (1 entity in total)
• Functional Keywords: arid, swi1, structural genomics, protein-dna interaction, dna binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: O14497
• Total number of polymer chains: 1
• Total formula weight: 13586.55

Authors

Kim, S.,Zhang, Z.,Upchurch, S.,Isern, N.,Chen, Y. (deposition date: 2003-12-22, release date: 2004-05-25, Last modification date: 2024-05-22)

Primary citation

Kim, S.,Zhang, Z.,Upchurch, S.,Isern, N.,Chen, Y.
Structure and DNA-binding sites of the SWI1 AT-rich interaction domain (ARID) suggest determinants for sequence-specific DNA recognition.
J.Biol.Chem., 279:16670-16676, 2004

PubMed Abstract: ARID (AT-rich interaction domain) is a homologous family of DNA-binding domains that occur in DNA-binding proteins from a wide variety of species, ranging from yeast to nematodes, insects, mammals, and plants. SWI1, a member of the SWI/SNF protein complex that is involved in chromatin remodeling during transcription, contains the ARID motif. The ARID domain of human SWI1 (also known as p270) does not select for a specific DNA sequence from a random sequence pool. The lack of sequence specificity shown by the SWI1 ARID domain stands in contrast to the other characterized ARID domains, which recognize specific AT-rich sequences. We have solved the three-dimensional structure of human SWI1 ARID using solution NMR methods. In addition, we have characterized nonspecific DNA binding by the SWI1 ARID domain. Results from this study indicate that a flexible, long, internal loop in the ARID motif is likely to be important for sequence-specific DNA recognition. The structure of the human SWI1 ARID domain also represents a distinct structural subfamily. Studies of ARID indicate that the boundary of DNA binding structural and functional domains can extend beyond the sequence homologous region in a homologous family of proteins. Structural studies of homologous domains such as the ARID family of DNA-binding domains should provide information to better predict the boundary of structural and functional domains in structural genomic studies.

PubMed: 14722072
DOI: 10.1074/jbc.M312115200

Experimental method

SOLUTION NMR