1RQ6

Solution structure of ribosomal protein S17E from Methanobacterium Thermoautotrophicum, Northeast Structural Genomics Consortium Target TT802 / Ontario Center for Structural Proteomics Target Mth0803

Summary for 1RQ6

• Entry DOI: 10.2210/pdb1rq6/pdb
• NMR Information: BMRB: 6028
• Descriptor: 30S ribosomal protein S17e (1 entity in total)
• Functional Keywords: alpha protein, structural genomics, protein structure initiative, psi, nesg, northeast structural genomics consortium, translation
• Biological source: Methanothermobacter thermautotrophicus
• Total number of polymer chains: 1
• Total formula weight: 7213.34

Authors

Wu, B.,Yee, A.,Huang, Y.J.,Ramelot, T.A.,Semesi, A.,Jung, J.W.,Edward, A.,Lee, W.,Kennedy, M.A.,Montelione, G.T.,Arrowsmith, C.H.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2003-12-04, release date: 2004-12-14, Last modification date: 2024-05-22)

Primary citation

Wu, B.,Yee, A.,Huang, Y.J.,Ramelot, T.A.,Cort, J.R.,Semesi, A.,Jung, J.W.,Lee, W.,Montelione, G.T.,Kennedy, M.A.,Arrowsmith, C.H.
The solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum: a structural homolog of the FF domain.
Protein Sci., 17:583-588, 2008

PubMed Abstract: The ribosomal protein S17E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. S17E is a 62-residue protein conserved in archaea and eukaryotes and has no counterparts in bacteria. Mammalian S17E is a phosphoprotein component of eukaryotic ribosomes. Archaeal S17E proteins range from 59 to 79 amino acids, and are about half the length of the eukaryotic homologs which have an additional C-terminal region. Here we report the three-dimensional solution structure of S17E. S17E folds into a small three-helix bundle strikingly similar to the FF domain of human HYPA/FBP11, a novel phosphopeptide-binding fold. S17E bears a conserved positively charged surface acting as a robust scaffold for molecular recognition. The structure of M. thermoautotrophicum S17E provides a template for homology modeling of eukaryotic S17E proteins in the family.

PubMed: 18218711
DOI: 10.1110/ps.073272208

Experimental method

SOLUTION NMR