1RPB

SOLUTION STRUCTURE OF RP 71955, A NEW 21 AMINO ACID TRICYCLIC PEPTIDE ACTIVE AGAINST HIV-1 VIRUS

Summary for 1RPB

• Entry DOI: 10.2210/pdb1rpb/pdb
• Descriptor: Tricyclic peptide RP 71955 (1 entity in total)
• Functional Keywords: hiv replication inhibitor, replication inhibitor
• Biological source: actinomycete Sp9440
• Total number of polymer chains: 1
• Total formula weight: 2185.53

Authors

Frechet, D.,Guitton, J.D.,Herman, F.,Faucher, D.,Helynck, G.,Monegier Du Sorbier, B.,Ridoux, J.P.,James-Surcouf, E.,Vuilhorgne, M. (deposition date: 1993-08-31, release date: 1994-01-31, Last modification date: 2024-06-05)

Primary citation

Frechet, D.,Guitton, J.D.,Herman, F.,Faucher, D.,Helynck, G.,Monegier du Sorbier, B.,Ridoux, J.P.,James-Surcouf, E.,Vuilhorgne, M.
Solution structure of RP 71955, a new 21 amino acid tricyclic peptide active against HIV-1 virus.
Biochemistry, 33:42-50, 1994

PubMed Abstract: The structure of RP 71955, a new tricyclic 21 amino acid peptide active against human immunodeficiency virus 1, was determined. Its amino acid composition was inferred from the results of fast atom bombardment mass spectrometry, nuclear magnetic resonance, Raman spectroscopy, and amino acid analysis. Its sequence could not be determined classically, using Edman degradation, given the lack of a free terminal NH2. It was deduced from the interpretation of interresidue nuclear Overhauser effects and confirmed by the sequencing of peptides obtained by limited chemical hydrolysis. It was found to be CLGIGSCNDFAGCGYAVVCFW. An internal amide bond between the NH2 of C1 and the gamma-COOH of D9 was observed, as well as two disulfide bridges, one between C1 and C13 and one between C7 and C19. The three-dimensional structure of RP 71955 was determined from nuclear magnetic resonance derived constraints using distance geometry, restrained molecular dynamics, nuclear Overhauser effect back calculation, and an iterative refinement using a full relaxation matrix approach. Analogies between the structure of RP 71955 and some functional domains of gp41, the transmembrane protein of human immunodeficiency virus 1, suggest hypotheses concerning the mode of action of RP 71955.

PubMed: 8286361
DOI: 10.1021/bi00167a006

Experimental method

SOLUTION NMR